The antibodies used for the immunoblot assays were: GCLC (Santa Cruz Biotech, #sc-390811), NQO1 (Sigma Prestige Antibodies, HPA007308), ACTIN (Sigma, A1978), NRF2 (Cell Signaling Technology, #12721), NRF1 (Cell Signaling Technology, #8052), YAP/TAZ (Cell Signaling, #8418) 4-HNE (Abcam, #ab46545) and Lamin A/C (Cell Signaling, #4777)
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You will need your cagrilintide 5mg lyophilized vial, a vial of bacteriostatic water (not sterile water, not saline), alcohol swabs, a mixing syringe (1mL or 3mL), and your dosing syringes (U-100 insulin syringes with 29-31 gauge needles)
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Lipid-induced insulin resistance in skeletal muscle: the chase for the culprit goes from total intramuscular fat to lipid intermediates, and finally to species of lipid intermediates
